One of the new separation methods for purifying biodiesel used in recent years is the membrane separation process. In the current research, purification of produced biodiesel was carried out using Polymeric Poly Ether Sulfone(PES) based membrane. The prepared PVP membranes was containing 1, 1.5, 2 and 3 wt.% of PVP concentration with 16 wt.% PES. as additive. Reported experimental determining the membrane flux and separation efficiency (glycerol rejection) membrane was modeled by Maxwell Model. The result, including penetration rate, theory graph and experimental data comparison for biodiesel and glycerin, and error graphs have been explained. Results showed that the experimental data’s have Compatibility with the model and, membrane with 2 percent PVP has provided more acceptable results.